KMID : 0379520020180030227
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Çѱ¹µ¶¼ºÇÐȸÁö 2002 Volume.18 No. 3 p.227 ~ p.232
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Comparison of Sensitivity Between Balb/c 3T3 Cell and HaCaT Cell by NRU Assay to Predict Skin Phototoxicity Potential
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Lee Jong-Kwon
Lee Eun-Hee Lee Sun-Hee
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Abstract
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In order to find out the appropriate in vitro method for high correlation with in vivo, we com-pared the sensitivities of phototoxicity (PT) in vitro method between in human keratinocytes, HaCaT cells and in 3T3 fibroblast cells derived from Balb/c mice. Both cells were exposed to six known phototoxic chemicals : promethazine, neutral red, chlortetracycline, amiodarone, bithionol, 8-methoxypsoralen, or non-phototoxic chemical, ALS (ammonium laureth sulfate) and then irradiated with 5 J/cm^2 of UVA. Cell viability (IC_{50} ) was measured by neutral red uptake (NRU) assay. The ratio of IC_{50} value of chemicals in the presence and absence of UVA was determined by the cut-off value. The phototoxic potential of test chemicals in NRU assay was determined by measuring the photoirriation factor (PIF) with a cut-off value of 5. In both 3T3 and HaCaT cells, all known phototoxic chemicals were positive (over 5 of PIF value), except that bithionol was found to be non-phototoxic to HaCaT cells, and ALS, non-phototoxic chemical was negative. These results suggest that Balb/c 3T3 cell was more sensitive than HaCaT cell to predict phototoxicity potential.
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KEYWORD
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3T3 fibroblast, HaCaT Cell, NRU, Phototoxicity, Sensitivity
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